Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of activating blood circulation and removing blood stasis on recurrence of alcoholic acute pancreatitis, and the possible underlying mechanism

Haicheng Dong¹, Weixing Ying², Shifeng Zhu²

¹Department of Traditional Chinese Medicine, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou 310012, Zhejiang Province, China; ²Department of Gastroenterology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, Zhejiang Province, China.

For correspondence:- Shifeng Zhu Email: doukemei268790@163.com

Accepted: 26 August 2023 Published: 30 September 2023

Citation: Dong H, Ying W, Zhu S. Effect of activating blood circulation and removing blood stasis on recurrence of alcoholic acute pancreatitis, and the possible underlying mechanism. Trop J Pharm Res 2023; 22(9):1849-1855 doi: 10.4314/tjpr.v22i9.11

© 2023 The authors.
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Abstract

Purpose: To determine the effect of activating blood circulation and removing blood stasis on the recurrence of alcoholic acute pancreatitis (AAP), and its possible mechanism.

Methods: Sixty healthy rats were selected and assigned to control, model and treatment groups (n =20/group). Treatment group was given Taohong Siwu decoction, while control and model groups were given abdominal injection of 0.9 % normal saline. Serum levels of amylase, pancreatic histopathological score, col ? content, mRNA expression levels of TGF-β 1, TβRII, and Smad3, as well as sPLA2 activity, were determined.

Results: Serum amylase, pancreatic histopathological score and col ? content were significantly reduced in treatment group, relative to model group, while relative mRNA expressions of TGF- β 1, TβRII and Smad3 at each time point were significantly lower than those in the model group (p < 0.05). Serum PLA2 activity and sPLA2 - π a mRNA expression were significantly lower than those in the model group (p < 0.05).

Conclusion: Activation of blood circulation and removal of blood stasis significantly prevents the relapse of AAP in rats through regulation of PLA2 activity and TGF-β1/TβRII/Smad3 signaling pathway. Cohort studies in humans would be required to confirm the application of Taohong Siwu decoction to prevent recurrence of AAP.

Keywords: Alcoholic acute pancreatitis, Recurrence, Blood circulation, PLA2, Blood stasis